Beam breaks actimeter12/27/2022 ![]() For example, this behavior can be influenced by exploratory drive ( i.e. The test is behavioral and can be quite variable as it is influenced by a multitude of external factors. However, the open field activity monitoring system also has significant challenges associated with it. ![]() Overall this makes the open field activity test a beneficial secondary or auxiliary outcome measure to use in preclinical trials. In addition, the open field activity measure, total distance traveled, mirrors the 6 min walk test, a clinical trial outcome measure, which focuses on submaximal exercise performance and quality of life 15,16. The open field activity assessment provides a different measure than muscle strength, which is commonly measured with grip strength, and it shows how drugs may affect other body systems (i.e. In particular, the open field is a useful tool for assessing locomotive impairment in animal models of neuromuscular disease 11,12 and the efficacy of therapeutic drugs that may improve locomotion and/or motor function 6,7,13,14. The test is also widely used to assess anxiety like and exploratory behaviors 8-10. The open field activity monitoring system comprehensively assesses locomotor and behavioral activity levels of mice, which can be correlated with locomotive function. In turn, this article is adapted from our previously published TREAT-NMD SOP 5. We have used this methodology to phenotype and test the therapeutic efficacy of numerous drugs in a variety of animal models of neuromuscular disease, including Lama2 dy-2J/J (Dy2J) mice, the animal model for congenital muscular dystrophy (CMD) 6,7. This SOP was previously validated 4 and published as a part of TREAT-NMD’s SOPs for Duchenne muscular dystrophy (DMD) animal models 5. As a member of the Congenital Muscle Disease Consortium, with the desire to conduct more rigorous preclinical studies, we share here our Standard Operating Procedure (SOP) for Open Field Activity. In particular, there is a need for rigorous methodologies that can be carried out in a standardized and blinded fashion, with validated, reproducible, and translatable outcome measures. With the current advances in drug development for neuromuscular disorders, there is an increasing need for well-designed preclinical trials. These discrepancies are often attributed to publication bias, overoptimistic conclusions, and poorly designed and executed pre-clinical studies that lead to irreproducible results 1-3. Numerous “promising” preclinical studies are published each year however, very few of the proposed interventions show positive results when moved to clinical trial. In addition, we provide recommendations for optimal study design when using open field activity in a preclinical trial.Īnimal models have been useful for learning about disease mechanisms, but their utility in predicting treatment efficacy in clinical trials has frequently been challenged 1-3. ![]() We provide a discussion of important considerations, typical results, data analysis, and detail the strengths and weaknesses of open field testing. Overall, this manuscript provides a well-tested and standardized open field activity SOP for preclinical trials in animal models of neuromuscular diseases. In addition, room temperature, humidity, lighting, noise, and even odor can affect assessment outcomes. However, open field activity monitoring is also associated with significant challenges: Open field activity measurements vary according to animal strain, age, sex, and circadian rhythm. In addition, measures such as total distance traveled mirror the 6 min walk test, a clinical trial outcome measure. It can also show how drugs may affect other body systems as well when used with additional outcome measures. The open field activity measurement provides a different measure than muscle strength, which is commonly assessed by grip strength measurements. ![]() It is a useful tool for assessing locomotive impairment in animal models of neuromuscular disease and efficacy of therapeutic drugs that may improve locomotion and/or muscle function. The open field activity monitoring system comprehensively assesses locomotor and behavioral activity levels of mice. ![]()
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